It has been found in all life form that protein lipidation, which is crucial in post-translational modifications (PTMs) that enable direct interaction with cell membranes. In the pattern of lipidation changing of membrane related proteins, the proteins have been detected in cancer and neurological disorders. It is a key determinant of its immunogenicity for lipidation at the N-terminal of recombinant outer surface protein A. lipid analysis methods www.creative-proteomics.com/pronalyse/lipidation-analysis.html