Biotin (Vitamin B7) is a vitamin essential for the maintenance of normal physiological functions in the animal organism. It plays an important role in the metabolism of glucose, amino acids and fatty acids in mammals as a component of four carboxylase coenzymes. There are five biotin-dependent carboxylases: methylcrotonyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase and two forms of acetyl-CoA carboxylase. biotin analysis method by hplc metabolomics.creative-proteomics.com/biotin-metabolism-service.htm